Study in SARS-CoV2 - infected mice may lay the groundwork for novel therapies to reduce the severity of COVID-19

Individuals who are immunocompromised are considered at higher risk for severe or longer disease with COVID-19. Understanding the systemic immune response is vital for research efforts to reduce its effects on multiple organs. A new study opens in new tab/window in SARS-CoV2-infected mice reported in The American Journal of Pathology opens in new tab/window, published by Elsevier, found lymphoid depletion lesions in the spleen that may form the groundwork for novel therapies to restore defective antigen-presenting cell (APC) functions in humans to trigger the cellular immune response and potentially reduce the severity of COVID-19.

The severity of COVID-19 varies considerably from no symptoms at all to severe disease with fatal complications. The risk of developing severe disease has been associated with dysregulated immune responses in patients. COVID-19 is known to impact not only the lungs but also non-target organs, leading to long-term complications and, in severe cases, multi-organ dysfunction. In particular, lymphopenia and lymphoid depletion in lymphoid tissues has been associated with poor disease outcomes.

Lead investigators Je Kyung Seong, DVM, PhD, Korea Mouse Phenotyping Center (KMPC), Seoul National University, and Jun Won Park, DVM, PhD, College of Biomedical Science, Kangwon National University, explained, “The mechanisms involved in the development of severe disease remain elusive, partially due to the limitations in evaluating immunologic factors of clinical samples, which could be affected by various circumstances, such as underlying diseases, age, sex, exposed environments, and eating habits.”

They added, “Investigating the factors underlying the risk of developing severe disease in terms of immunodynamics under well-controlled infectious conditions based on animal models is essential to understanding the mortality and immunologic factors that contribute to disease severity. Therefore, gaining insight into the systemic immune response, beyond just the lung-related lesions, is vital for research efforts focused on reducing the effects of COVID-19 on multiple organs and lessening the long-term consequences.”

In this study, human angiotensin-converting enzyme 2 (K18-hACE2) transgenic mouse models susceptible to SARS-CoV-2 infection, the strain of coronavirus that causes COVID-19, were used to investigate the characteristics and determinants of lethality associated with the lymphoid depletion observed in COVID-19.

Dr. Seong noted, “The K18-hACE2 mouse model is extensively employed in preclinical trials for the development of COVID-19 treatments and vaccines. Our research group has performed numerous preclinical studies using this model. During these experiments, we discovered a strong correlation between the mice's mortality rate, fatal brain infections, and lymphoid depletion observed in their spleens.”

Dr. Park added, “As similar splenic lesions are also found in patients with severe COVID-19, it is crucial to characterize lymphoid depletion in this lethal mouse model. Doing so will enable a better understanding of the factors contributing to COVID-19 patient mortality and aid in the development of effective therapies to mitigate these outcomes.”

The infected mice exhibited a diverse range of disease severity in terms of weight, temperature, lung pathology, spleen pathology, and brain infection. The correlation between these parameters was then analyzed to define the lethality induced by COVID-19 in the mouse model. The results indicated that, in addition to lung lesions, brain viral load is a major determinant of mortality in this mouse model.

Caption: A comparison of severity and lymphoid depletion in mice infected with Wuhan, Delta, and Omicron SARS-CoV-2 and Influenza A. DCs, dendritic cells; MHC, major histocompatibility complex; APCs, antigen presenting cells (Credit: The American Journal of Pathology).

The results collectively demonstrate that lymphoid depletion lesions observed in the spleen serve as a crucial factor reflecting mortality rates. Lymphoid depletion accompanied by reduced APC function was a specific feature observed in the Wuhan and Delta types of SARS-CoV-2 but not in other infections such as Omicron and influenza A and had the greatest prognostic value for disease severity in mice infected with the Wuhan and Delta variants.

Dr. Seong commented, “Thus, we demonstrated that lymphoid depletion associated with suppressed APC function characterizes the lethality of COVID-19 in mouse models.”

Dr. Park observed, “Our study may form the groundwork for novel therapies to restore defective APC functions in patients with COVID-19 and possibly prevent its severe progression by enhancing APC functionality.”

Accordingly, the investigators recommend evaluating the spleen in K18-hACE2 mice to assess vaccine and therapeutic efficacy in preclinical testing.

---

Notes for editors

The article is “Murine Coronavirus Disease 2019 Lethality Is Characterized by Lymphoid Depletion Associated with Suppressed Antigen-Presenting Cell Functionality,” by Yu Jin Lee, Sang Hyeok Seok, Na Yun Lee, Hee Jin Choi, Yoon Woo Lee, Hee Jung Chang, Ji-Yeon Hwang, Da In On, Hyun Ah Noh, Su-Bin Lee , Ho-Keun Kwon, Jun-Won Yun, Jeon-Soo Shin, Jun-Young Seo, Ki Taek Nam, Ho Lee, Ho Young Lee, Jun Won Park, and Je Kyung Seong (https://doi.org/10.1016/j.ajpath.2023.03.008 opens in new tab/window). It appears online in advance of The American Journal of Pathology, volume 193, issue 7 (July 2023), published by Elsevier opens in new tab/window.

The article is openly available at https://ajp.amjpathol.org/article/S0002-9440(23)00119-0/fulltext opens in new tab/window.

Full text of the article is also available to credentialed journalists upon request. Contact Eileen Leahy at +1 732 238 3628 or [email protected] opens in new tab/window to request a PDF of the article. To request an interview with the authors please contact Je Kyung Seong at [email protected] opens in new tab/window or Jun Won Park at [email protected] opens in new tab/window.

About The American Journal of Pathology 

The American Journal of Pathology opens in new tab/window, the official journal of the American Society for Investigative Pathology opens in new tab/window, published by Elsevier, seeks high-quality original research reports, reviews, and commentaries related to the molecular and cellular basis of disease. The editors will consider basic, translational, and clinical investigations that directly address mechanisms of pathogenesis or provide a foundation for future mechanistic inquiries. Examples of such foundational investigations include data mining, identification of biomarkers, molecular pathology, and discovery research. High priority is given to studies of human disease and relevant experimental models using molecular, cellular, and organismal approaches. https://ajp.amjpathol.org opens in new tab/window

About Elsevier

As a global leader in scientific information and analytics, Elsevier helps researchers and healthcare professionals advance science and improve health outcomes for the benefit of society. We do this by facilitating insights and critical decision-making with innovative solutions based on trusted, evidence-based content and advanced AI-enabled digital technologies.

We have supported the work of our research and healthcare communities for more than 140 years. Our 9,500 employees around the world, including 2,500 technologists, are dedicated to supporting researchers, librarians, academic leaders, funders, governments, R&D-intensive companies, doctors, nurses, future healthcare professionals and educators in their critical work. Our 2,900 scientific journals and iconic reference books include the foremost titles in their fields, including Cell Press, The Lancet and Gray’s Anatomy.

Together with the Elsevier Foundation opens in new tab/window, we work in partnership with the communities we serve to advance inclusion and diversity in science, research and healthcare in developing countries and around the world.

Elsevier is part of RELX opens in new tab/window, a global provider of information-based analytics and decision tools for professional and business customers. For more information on our work, digital solutions and content, visit www.elsevier.com.